Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6492071 | Journal of Biotechnology | 2012 | 8 Pages |
Abstract
⺠A new method to over-express and purify homologous proteins from Streptococcus pneumoniae. ⺠Combined use of the plasmid pMU1328 and the pneumococcal promoter P96. ⺠Expression of secreted, membrane or cell wall proteins, wild type or mutant. ⺠Concurrent assessment of protein functionality and purification. ⺠Homologous pneumococcal RrgBHis purification and characterization.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Morena Lo Sapio, Markus Hilleringmann, Michèle A. Barocchi, Monica Moschioni,