| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6492078 | Journal of Biotechnology | 2012 | 8 Pages |
Abstract
⺠Accell siRNA is a new type of naked siRNA without requiring transfection reagents. ⺠We indicated a gene silencing of adult rat brain by Accell siRNA for the first time. ⺠The Accell siRNA was specifically incorporated into neuron, not into glia. ⺠Two proteins resulted in knockdown in wide regions of brain by the procedure. ⺠The presented method enables an in vivo gene silencing of brain easily and quickly.
Keywords
FAMGFAPDABGAPDHCNPDAPIi.c.v.3,3-diaminobenzidine4′,6-diamidino-2-phenylindoleIba-1RNA interferenceSmall interfering RNAsiRNAImmunofluorescenceIn vivo deliveryCNScentral nervous systemRat brainionized calcium binding adaptor molecule-1Glial fibrillary acidic proteinglyceraldehydes-3-phosphate dehydrogenase
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Hidemitsu Nakajima, Takeya Kubo, Yuko Semi, Masanori Itakura, Mitsuru Kuwamura, Takeshi Izawa, Yasu-Taka Azuma, Tadayoshi Takeuchi,