| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6492084 | Journal of Biotechnology | 2012 | 8 Pages |
Abstract
⺠This report describes for the first time an efficient protocol for production and purification of plant catalase in E. coli system. ⺠Thioredoxin and molecular chaperone, the ribosomal Trigger Factor and low temperature treatment are necessary for enhancing the solubility of recombinant rice plant catalase. ⺠Supplementation of a heme precursor (ALA) is essential for heme incorporation into the newly synthesized recombinant catalase.
Keywords
dithioerythritolALADTEPVDFIPTGHydrogen peroxideEDTAEthylenediaminetetraacetic acidδ-aminolevulinic acidEscherichia colisodium dodecylsulphate-polyacrylamide gel electrophoresisSDS-PAGEisopropyl β-D-1-thiogalactopyranosideRecombinant expressionLuria BertaniH2O2polymerase chain reactionPCRPolyvinylidene fluoride
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Mamata Ray, Panchanand Mishra, Priyanka Das, Surendra Chandra Sabat,