| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6492114 | Journal of Biotechnology | 2012 | 11 Pages |
Abstract
⺠Twelve enzymes expressed and purified for reconstituting fermentative glycolysis. ⺠Simple assays are described for each glycolytic enzyme. ⺠In vitro fermentation in isolation recycled ATP with glucose as substrate. ⺠The ratio of glucokinase to phosphofructokinase was critical for this process. ⺠Engineered pyruvate decarboxylase allowed faster ethanol production.
Keywords
DHAPG6PenolaseGPieNOPGMPEPTEPTPI1,3-bisphosphoglyceratePFKPGKF6PFBP3PGTPPCFPSADHTriose-phosphate isomeraseDTTPDCGAPDHFBA2PG2-phosphoglycerate3-phosphoglycerate6-Phosphofructokinasepiperazine-N,N′-bis(2-ethanesulfonic acid)Adenosine TriphosphateAlcohol dehydrogenaseCell-freeFermentationTriethylphosphateThiamine pyrophosphatedihydroxyacetone phosphatePyruvate decarboxylasedithiothreitolCell-free protein synthesisGAP31P NMR spectroscopyfructose 1,6-bisphosphateFructose 6-phosphateFructose-1,6-bisphosphate aldolaseinorganic phosphatephosphoglycerate mutasephosphoglycerate kinasePipespyruvate kinasePYKGlucoseGlucose 6-phosphateGlucose-6-phosphate isomeraseGlucokinaseGlkglyceraldehyde-3-phosphate dehydrogenaseglyceraldehyde 3-phosphate
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Bradley J. Stevenson, Jian-Wei Liu, Philip W. Kuchel, David L. Ollis,