| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6492155 | Journal of Biotechnology | 2012 | 9 Pages |
Abstract
⺠Previous single mutants of transketolase improved activity on new substrates. ⺠Recombination to form double mutants led to critical loss of functional expression. ⺠Mutated sites were found to be in a structural network of co-evolved residues. ⺠The network was re-adapted around previous mutants for kinetic synergy and functional expression.
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Authors
John Strafford, Panwajee Payongsri, Edward G. Hibbert, Phattaraporn Morris, Sukhjeet S. Batth, David Steadman, Mark E.B. Smith, John M. Ward, Helen C. Hailes, Paul A. Dalby,