Fabrication of polyphenol biosensor based on laccase immobilized on copper nanoparticles/chitosan/multiwalled carbon nanotubes/polyaniline-modified gold electrode

► Covalent immobilization of Ganoderma sp. laccase onto copper nanoparticles (CuNP's)/chitosan (CHIT)/carboxylated multiwalled carbon nanotube (cMWCNT)/polyaniline (PANI)-modified gold (Au) electrode for amperometric polyphenol biosensor. ► Optimum response within 4 s at pH 6.0 (0.1 M acetate buffer) and 35 °C at 50 mV s−1. ► 300 uses of enzyme electrode over a period of 7 months, when stored at 4 °C.