| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6492219 | Journal of Biotechnology | 2011 | 7 Pages |
Abstract
Transfection efficiencies of up to 33% with pCEP4 and 98% with pMAX were reached. Additionally immobilisation on microcarriers was used to retain the cells during cultivation, thus allowing media replacement and prolonging cultivation time from one to two weeks with continuous expression of the recombinant protein.
Keywords
PBSr-proteinsTGENS0oriPBHKHEK293eGFPRPMPEIGFP3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromideD/HMTTChoTransient gene expressionProtein productionChinese hamster ovary cellsbaby hamster kidney cellsMicrocarrierGreen fluorescence proteinenhanced green fluorescence proteinRecombinant proteinsPolyethyleniminerotations per minutehuman embryo kidney
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Lukas Fliedl, Christian Kaisermayer,