| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6492263 | Journal of Biotechnology | 2010 | 9 Pages |
Abstract
Dimethyl sulfoxide at 1.5% concentration has been found to increase the levels of L1/Ig5-6 and the L1 ectodomain in the Sf9 and Tn cells, without affecting cell viability nor protein integrity. Furthermore, the N-glycan composition of L1/Ig5-6 was not affected by dimethyl sulfoxide, with only a slight increase in the percentage of the minor high-mannose-type structures.
Keywords
MALDI/TOF-MSdHexHexNAcFucFucosecell adhesion molecule L1Peptide-N-glycosidase FGlcNAcPNGase FHPAEC-PADDMSOendo-β-N-acetylglucosaminidase HN-acetylhexosamineN-glycosylationEDTAmatrix-assisted laser desorption/ionization time-of-flight mass spectrometryEndo Himmunoglobulin deoxyhexoseDimethyl sulfoxideInsect cellsMannoseManN-acetylglucosamineHexHexoseGalGalactose
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Ricardo Gouveia, Sebastian Kandzia, Harald S. Conradt, Júlia Costa,