Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6494274 | Metabolic Engineering | 2016 | 31 Pages |
Abstract
The development of lignocellulose as a sustainable resource for the production of fuels and chemicals will rely on technology capable of converting the raw materials into useful compounds; some such transformations can be achieved by biological processes employing engineered microorganisms. Towards the goal of valorizing the hemicellulose fraction of lignocellulose, we designed and validated a set of pathways that enable efficient utilization of pentoses for the biosynthesis of notable two-carbon products. These pathways were incorporated into Escherichia coli, and engineered strains produced ethylene glycol from various pentoses, including simultaneously from D-xylose and L-arabinose; one strain achieved the greatest reported titer of ethylene glycol, 40Â g/L, from D-xylose at a yield of 0.35Â g/g. The strategy was then extended to another compound, glycolate. Using D-xylose as the substrate, an engineered strain produced 40Â g/L glycolate at a yield of 0.63Â g/g, which is the greatest reported yield to date.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Brian Pereira, Zheng-Jun Li, Marjan De Mey, Chin Giaw Lim, Haoran Zhang, Claude Hoeltgen, Gregory Stephanopoulos,