Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6494579 | Metabolic Engineering | 2014 | 10 Pages |
Abstract
During single-cell analysis of cells expressing the pH sensitive fluorescent probe pHluorin, pHluorin fluorescence was gradually lost from the cells during D-xylonate production, as expected for cells with decreasing intracellular pH. However, in the presence of D-xylose, only 9% of cells expressing xylB lost pHluorin fluorescence within 4.5Â h, whereas 99% of cells co-expressing xylB and xylC lost fluorescence, a large proportion of which also lost vitality, during this interval. Loss of vitality in the presence of D-xylose was correlated to the extracellular pH, but fluorescence was lost from xylB and xylC expressing cells regardless of the extracellular condition.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Yvonne NygÃ¥rd, Hannu Maaheimo, Dominik Mojzita, Mervi Toivari, Marilyn Wiebe, Orna Resnekov, C. Gustavo Pesce, Laura Ruohonen, Merja Penttilä,