Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6494625 | Metabolic Engineering | 2014 | 7 Pages |
Abstract
Fatty acid short-chain esters (FASEs) are biodiesels that are renewable, nontoxic, and biodegradable biofuels. A novel approach for the biosynthesis of FASEs has been developed using metabolically-engineered E. coli through combination of the fatty acid and 2-keto acid pathways. Several genetic engineering strategies were also developed to increase fatty acyl-CoA availability to improve FASEs production. Fed-batch cultivation of the engineered E. coli resulted in a titer of 1008Â mg/L FASEs. Since the fatty acid and 2-keto acid pathways are native microbial synthesis pathways, this strategy can be implemented in a variety of microorganisms to produce various FASEs from cheap and readily-available, renewable, raw materials such as sugars and cellulose in the future.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Daoyi Guo, Jing Zhu, Zixin Deng, Tiangang Liu,