| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6494648 | Metabolic Engineering | 2014 | 12 Pages |
Abstract
⺠“Difficult-to-express” proteins consistently yield low expression from mammalian cells. ⺠Low protein secretion can stem from faulty cleavage and precipitation in degradation organelles. ⺠Expression of translational arrest and secretion pathway components restores proper secretion. ⺠Secretion pathway engineering also increases expression of an easy-to-express immunoglobulin. ⺠Metabolic engineering thus resolves CHO cell protein processing and secretion bottlenecks.
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Authors
Valérie Le Fourn, Pierre-Alain Girod, Montse Buceta, Alexandre Regamey, Nicolas Mermod,