Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6494849 | New Biotechnology | 2018 | 29 Pages |
Abstract
Clonal propagation plays a critical integral role in the growth and success of a global multi-billion dollar horticulture industry through a constant supply of healthy stock plants. The supply chain depends on continuously improving the micropropagation process, thus, understanding the physiology of in vitro plants remains a core component. We evaluated the influence of exogenously applied cytokinins (CKs, N6-benzyladenineâ¯=â¯BA, isopentenyladenineâ¯=â¯iP, meta-topolinâ¯=â¯mT, 6-(3-hydroxybenzylamino)-9-(tetrahydropyran-2-yl)purineâ¯=â¯mTTHP) in Murashige and Skoog (MS)-supplemented media on organogenic response and accumulation of endogenous CK and indole-3-acetic acid (IAA) metabolites. The highest shoot proliferation (30 shoots/explant) was obtained with 20â¯Î¼MâmT treatment. However, the best quality regenerants were produced in 10â¯Î¼MâmT treatment. Rooting of Amelanchier alnifolia in vitro plantlets was observed at the lowest CK concentrations, with the highest root proliferation (3 roots/explant) in 1â¯Î¼M mTTHP regenerants. Similar to the organogenic response, high levels of endogenous bioactive CK metabolites (free bases, ribosides, and nucleotides) were detected in mT and mTTHP-derived regenerants. The level of O-glucosides was also comparatively high in these cultures. All CK-treated plants had high levels of endogenous free IAA compared to the control. This may suggest an influence of CKs on biosynthesis of IAA.
Keywords
PPFMeta-topolinDHZN6-IsopentenyladenineIPRMTRtZRiP9GESIDHZRcZRcZOGcis-zeatinDihydrozeatin-O-glucosideBA9GN6-benzyladeninemTOGIAAOTRMurashige and Skoog mediumPTrindole-3-acetic acidOrganogenesisAuxinsBARtrans-ZeatindihydrozeatinMicropropagationcytokininEndogenous cytokininsphotosynthetic photon flux densityUltra-high performance liquid chromatographykinetin
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Mack Moyo, Adeyemi O. Aremu, Lenka PlaÄková, Lucie PlÃhalová, AleÅ¡ PÄnÄÃk, OndÅej Novák, Jan Holub, Karel Doležal, Johannes Van Staden,