Kinetic study on the enzymatic esterification of octanoic acid and hexanol by immobilized Candida antarctica lipase B

- Effects of substrate, catalyst amount and biphasic reaction medium were studied.
- Ping-Pong bi-bi model with twin substrate inhibition was used at initial rates.
- Possible ester inhibition and catalyst denaturation by substrates were investigated.
- Reverse reaction was considered and a reversible kinetic model was proposed.
- The intrinsic kinetic model was validated by experimental progress-curve data.