Novelty of immobilized enzymatic synthesis of 3-ethyl-1,3-oxazolidin-2-one using 2-aminoalcohol and dimethyl carbonate: Mechanism and kinetic modeling of consecutive reactions

- Synthesis of 3-ethyl-1,3-oxazolidin-2-one using 2-aminoalochol and dimethyl carbonate.
- Among 8 enzymes, Candida antarctica lipase B (Novozyme 435) was the best catalyst.
- First detailed report on modeling of two-step enzyme catalyzed consecutive reaction.
- Kinetic constants and activation energy were determined for each step.
- Protocol extended to different 2-aminoalochols to prepare a series of oxazolidinones.