Article ID Journal Published Year Pages File Type
6531191 Journal of Molecular Catalysis B: Enzymatic 2014 7 Pages PDF
Abstract

- A meso-2, 3-butanediol dehydrogenase from Bacillus subtilis was expressed in E. coli and characterized.
- Amino acid sequence alignment revealed a mutation from Asp to Gly at site 194.
- The substitution maintained substrate-binding ability of the enzyme but severely weaken its catalytic activity.
- Structural modifications of the enzyme might be the major reason causing the lack of enzyme activity.
Related Topics
Physical Sciences and Engineering Chemical Engineering Catalysis
Authors
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