Biochemical characterization of unusual meso-2,3-butanediol dehydrogenase from a strain of Bacillus subtilis

- A meso-2, 3-butanediol dehydrogenase from Bacillus subtilis was expressed in E. coli and characterized.
- Amino acid sequence alignment revealed a mutation from Asp to Gly at site 194.
- The substitution maintained substrate-binding ability of the enzyme but severely weaken its catalytic activity.
- Structural modifications of the enzyme might be the major reason causing the lack of enzyme activity.