Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6531214 | Journal of Molecular Catalysis B: Enzymatic | 2014 | 8 Pages |
Abstract
- An automated purification method in less than 6Â h was developed.
- According to silver stained SDS-PAGE PepX was purified to homogeneity.
- The kinetics of PepX with different original tripeptides was studied for the first time.
- The amino acids at position P2 and P1â² showed a strong influence on the kinetic parameters.
- Original tripeptides possessed lower Km values than artificial chromogenic peptides.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Timo Stressler, Thomas Eisele, Bertolt Kranz, Lutz Fischer,