| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6531276 | Journal of Molecular Catalysis B: Enzymatic | 2013 | 6 Pages |
Abstract
- kcat/Km of Q105R is equal to native cmFDH and this mutant increase the T0.5 value as 6 °C.
- Y160E has higher kcat/Km (0.35Â sâ1Â mMâ1) than that of native (0.24Â sâ1Â mMâ1).
- The Tm value was increased nearly 3 °C for N147R and Q105.
- The most significant increase in Tm was observed for the N187E as 6 °C.
- H13E, Y160E, Y160R and Y302R showed on average of 6Â kcal/mol destabilizing effect.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Emel B. Ordu, Richard B. Sessions, Anthony R. Clarke, Nevin Gül Karagüler,