| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6531323 | Journal of Molecular Catalysis B: Enzymatic | 2013 | 7 Pages |
Abstract
- A beta-glucosidase is in vivo biotinylated and subsequently immobilized directly from cell lysate on streptavidin coated magnetic particles.
- The high affinity and strong interaction between biotin and streptavidin enables simultaneous purification and immobilization.
- Immobilized beta-glucosidase displays higher thermal stability, at 50 °C, compared to free enzyme.
- It is possible to recycle the immobilized enzyme at least 9 recycles and retain 89% of its initial activity.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Johan Alftrén, Kim Ekelund Ottow, Timothy John Hobley,