| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 6531339 | Journal of Molecular Catalysis B: Enzymatic | 2013 | 6 Pages |
Abstract
- Bivalent ion binding to the calcium-binding site in mannanase enhanced thermal stability.
- Calcium-binding site deletion mutants did not bind calcium, manganese, and magnesium ions.
- Enhancement of thermal stability was related to the association constant Ka.
- Ka comprising negative enthalpy and low entropy was suitable for bivalent ion binding.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Yuya Kumagai, Kayoko Kawakami, Misugi Uraji, Tadashi Hatanaka,