Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6601471 | Electrochemistry Communications | 2013 | 4 Pages |
Abstract
Electrochemical impedance spectroscopy was evaluated for the label free detection of MCF-7 cancer cell in which c-erbB-2 receptor is overexpressed on the cell surfaces. Anti-c-erbB-2, used as a specific antibody, was immobilized on electrogenerated polypyrrole-NHS on electrodes via covalent linking. The polymer formation, the grafting of the antibody, and the recognition event with the cancer cells using MCF-7 as a model cell line, were characterized by using cyclic voltammetry and fluorescence microscopy. The impedimetric sensor showed high sensitivity from 100 to 10 000 cell/mL without needing any labeling step and represents an efficient transduction method for cell selective detection.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
Basak Seven, Mariem Bourourou, Kamal Elouarzaki, Jean François Constant, Chantal Gondran, Michael Holzinger, Serge Cosnier, Suna Timur,