Synthesis of curcumin loaded polymeric nanoparticles from crab shell derived chitosan for drug delivery

In this study, chitosan was derived from crab shell using two concentrations of hydrochloric acid i.e.1.5 M and 2 M HCl during demineralisation step. The obtained chitosan was utilised for synthesis of polymeric nanoparticles using sodium tripolyphosphate and barium chloride as chelators. The nanoparticles were encapsulated with hydrophobic curcumin and analysed for drug delivery invitro. Also, the synthesized nanoparticles were characterised by FTIR, SEM and AFM analysis and subjected for drug encapsulation efficiency, invitro drug release kinetics and controlled drug delivery studies in vitro against Pseudomonas aeruginosa. Barium chloride was found to produce spherical shaped drug loaded nanoparticles of size below 500 nm.