| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 69457 | Journal of Molecular Catalysis B: Enzymatic | 2016 | 5 Pages |
•CALB was immobilized on magnetic PUU NPs by interfacial polymerization.•Immobilization of CALB and the support synthesis were conducted simultaneously.•Increasing the content of CALB increased the particle diameter.•Immobilized CALB showed higher esters conversion.
In this work Candida antarctica lipase B (E.C: 3.1.1.3), was immobilized on magnetic poly(urea-urethane) nanoparticles (MNPs-PUU) in a single-step during the interfacial miniemulsion polymerization. Transmission electron microscopy images showed the morphology of synthesized magnetic nanoparticles encapsulated in poly(urea-urethane) nanoparticles and fluorescence microscopy images confirmed the enzyme immobilization onto MNPs-PUU. After the immobilization process, the immobilized enzyme on support (MNPs-PUU) was attracted by an external magnetic field and used as biocatalyst for the synthesis of esters ethyl oleate, geranyl propionate and geranyl oleate. Ester conversions above 85% were obtained for all systems based on the free fatty acids contents measured by titration until pH 11.
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