Efficient resveratrol production by immobilized β-glucosidase on cross-linked chitosan microsphere modified by l-lysine

•β-Glucosidase was immobilized on chitosan microsphere to produce resveratrol from enzymatic hydrolysis of polydatin.•The condition for the immobilization was optimized.•The characterization of the immobilized enzyme was carried out.•The activity of the immobilized enzyme remained 92.2 ± 0.9% after continuous hydrolysis for 8 h.

Resveratrol and its glycosidic derivative polydatin are the major stilbenes in Polygonum cuspidatum, a most widely used traditional herbal medicine. To produce resveratrol via enzymatic hydrolysis of polydatin, β-glucosidase was immobilized on cross-linked chitosan microspheres modified by l-lysine. The condition of enzyme immobilization was optimized, and the characterization of the immobilized enzyme was carried out. Km value of immobilized β-glucosidase was calculated as 47.7 ± 0.7 mM, and Vmax value was 34.6 ± 0.5 U/mg. Under optimized condition, which was 45 °C, pH 6.0, with the initial polydatin concentration of 2.0%, and the flow rate of 1.0 mL/min, the overall hydrolysis yield could reach 85.8 ± 0.6%, and the activity of the immobilized enzyme remained 92.2 ± 0.9% after continuous hydrolysis for 8 h. Enzymatic hydrolysis of polydatin extracted from P. cuspidatum is a promising method to increase the supply of resveratrol in pharmaceutical markets worldwide, which also showed a new application of immobilized β-glucosidase in biotechnological industry.

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