| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 69764 | Journal of Molecular Catalysis B: Enzymatic | 2014 | 9 Pages |
•A simple method for purification of c9,t11 CLA.•An esterification of CLA isomers catalyzed by Candida cylindracea lipase.•The effect of various parameters on isomeric excess of c9,t11 CLA.•Esterification was affected mostly by type of a solvent and alcohol substrate.•Two-step purification with crystallization and bi-phasic lipase esterification.
A method for purification of cis-9,trans-11 isomer of conjugated linoleic acid (CLA) is described. Lipase from Candida cylindracea (CCL) was highly selective towards cis-9,trans-11 isomer and was chosen to catalyze esterification of a mixture of two CLA isomers. The effect of various parameters on the esterification process of an almost equimolar mixture of cis-9,trans-11 (44.0%), trans-10,cis-12 (42.5%) and other CLA isomers (13.5%) was studied. The influence of alcohol substrate, CLA/alcohol molar ratio, lipase amount, reaction temperature, pH and time of the reaction on the total esterification and cis-9,trans-11 isomer purity in ester fraction were examined. The highest isomeric excess of cis-9,trans-11 isomer on trans-10,cis-12 CLA and good esterification degree was observed when the esterification condition were as follows: CLA/2-propanol molar ratio, 1:5; water, 200% wt of CLA; CCL, 24 μg of CCL/mg CLA; temperature 30 ̊C; time of reaction 6 h and when mixture enriched in cis-9,trans-11 CLA was used as a substrate for esterification. A preparative scale purification combining crystallization and one step enzymatic process were made to afford purified cis-9,trans-11 (94.2%) with total recovery of 31%.
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