Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
6978842 | Colloids and Surfaces A: Physicochemical and Engineering Aspects | 2015 | 8 Pages |
Abstract
- We report hybridization of DNA and RNA to a surface-bound DNA probe derived from TMV using SPR.
- For both RNA and DNA, the melting temperatures were â¼20 °C lower for solid state than in solution.
- Short oligomeric RNA targets may require measurement temperatures below room temperature.
- The melting temperature of the DNA:RNA hybrid was 17 °C lower than DNA:DNA duplex in the solid state.
- Hybrid melting temperatures are critical variables for biosensor oligonucleotide analysis.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Colloid and Surface Chemistry
Authors
Jing C. Zhou, Bob Feller, Bill Hinsberg, Geeta Sethi, Paul Feldstein, Joshua Hihath, Erkin Seker, Maria Marco, Andre Knoesen, Robert Miller,