Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
69866 | Journal of Molecular Catalysis B: Enzymatic | 2012 | 5 Pages |
The present work focuses on the oleic acid epoxide production by using PSCI Amano Lipase as biocatalyst in the reaction. An experimental design (central composite design – CCD) adopting surface response was applied to this purpose. Reactions were performed in a shaker equipment and different variables were investigated, such as temperature (25–55 °C), enzyme load (10–20 wt% of oleic acid mass), hydrogen peroxide load (0.1–0.2%) and reaction time. PSCI-Amano enzyme showed its best behavior as biocatalyst after 3 h of reaction at 55 °C, 10% enzyme load, 0.2% hydrogen peroxide and, applying 150 rpm as stirring. On these conditions, the epoxide yield was around 88%.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► We proposed oleic acid epoxidation by PSCI-Amano lipase under shaking to obtain epoxystearic acid. ► Experimental design (central composite design) surface response was applied. ► We considered temperature, enzyme content, peroxide concentration. ► Good yields were achieved in shorter enzyme load and higher temperature. ► Mathematic model proposed suggested a satisfactorily representation of the process.