| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 69900 | Journal of Molecular Catalysis B: Enzymatic | 2012 | 4 Pages |
The aim of this work was to investigate innovative reaction media such as ionic liquids for enzymatic phospholipids transformations in order to get a higher selectivity in the enzymatic transphosphatidylation reactions of natural phosphatidylcholine catalyzed by phospholipase D (PLD) from Streptomyces PMF. The stability and the activity of the PLD in the ionic liquid [BMIM][PF6] were tested. This ionic liquid used as a cosolvent in the synthesis of phosphatidylserine lead surprisingly to the almost quantitative suppression of unwanted hydrolytic side reaction, thus providing a great improvement in the final product purification.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► We investigate the use of ionic liquids in phospholipids transformations. ► A hydrophobic ionic liquid enhances enzyme selectivity. ► Hydrolysis activity of phospholipase D is inhibited by the presence of a hydrophobic ionic liquid.
