| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 69906 | Journal of Molecular Catalysis B: Enzymatic | 2012 | 4 Pages |
alpha-Linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) have been subjected to esterification with ethanol in presence of lipase B from Candida antarctica (Novozym 435®), in solvent free condition. The use of alcohol donors triethyl orthoformate (TEOF) or diethyl carbonate (DEC) instead of free ethanol, allowed working in irreversible esterification conditions and ALA-, EPA- and DHA-ethyl esters were obtained in quantitative yields.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Irreversible biocatalyzed esterification of n-3 PUFAs has been obtained by triethyl orthoformate or diethyl carbonate as alcohol donors. ► Preparation in high yields of n-3 PUFA ethyl esters. ► Solvent free biocatalyzed esterification of fatty acids.
