| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 70047 | Journal of Molecular Catalysis B: Enzymatic | 2012 | 7 Pages |
Mesoporous silica nanotubes and aminopropyl-grafted mesoporous silica nanotubes were prepared as supports to immobilize lipase from Candida sp. 99-125(ClLip) by physical adsorption. The immobilization conditions were investigated. Moreover, immobilized lipases on both kinds of supports were employed to catalyze olive oil hydrolization and resolution of 1-phenylethanol by esterification. The results showed that the hydrolization activity of the lipase immobilized on aminopropyl-grafted mesoporous silica nanotubes was almost twice of that on mesoporous silica nanotubes. In addition, the resolution of 1-phenylethanol catalyzed by the former catalyst also increased 22%. Circular dichroism spectra revealed a reduction of α-helix and an increase of β-sheet when lipase was adsorbed on aminopropyl-grafted mesoporous silica nanotubes, which suggested that parts of α-helix were extended and reformed to be β-sheet.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Two silica nanotubes were prepared as carriers to immobilize lipase physically. ► These two carriers and the subsequent immobilized lipases were compared. ► Aminopropyl-modification of the carrier doubled the activity of immobilized lipase. ► The resolution of 1-phenylethanol catalyzed by NH2-MSNTs-lipase achieved 22% more. ► Circular dichroism spectra showed structure changes of lipase after immobilization.
