| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 70145 | Journal of Molecular Catalysis B: Enzymatic | 2013 | 5 Pages |
Erucic acid enrichment from crambe oil was accomplished in a recirculated packed bed (RPBR) reactor via ethanolysis using Novozym 435 lipase from Candida antarctica as a biocatalyst. The content of erucic acid of the crambe oil used was 59.8 mol% The erucic acid was located predominantly in the sn-1,3 position of the triacylglycerol of the crambe oil. Novozym 435 used in this study has selectivity toward fatty acids in sn-1,3 position of triglyceride in excess ethanol. The effects of reaction temperature, molar ratio, and residence time of the substrate in RPBR on the enrichment of erucic acid as a function of reaction time were studied. The optimal temperature, molar ratio (crambe oil to ethanol), and residence time of the substrate in RPBR were 45 °C, 1:60, and 4 min, respectively.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Erucic acid enrichment was produced from crambe oil by ethanolysis using recirculated packed bed reactor. ► Novozym 435 lipase exhibited high selectivity at the sn-1,3 position of crambe oil triacylglycerol for ethanolysis. ► Pumping speed of recirculated packed bed reactor affected significantly the reaction for enrichment of erucic acid.
