Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
70255 | Journal of Molecular Catalysis B: Enzymatic | 2012 | 6 Pages |
Immobilized lipase has the potential to be the catalyst of choice for biodiesel production since it is efficient, effective, and environmentally friendly; however, the stability and activity of lipase must be addressed before enzymatic biodiesel production processes can be industrially accepted. This study investigates an enzyme immobilization procedure that immobilizes lipase in a sol–gel supported on diatomaceous earth (Celite® R632), and determines its potential for biodiesel production in terms of achievable conversion and apparent stability. Four immobilized materials (lipase sol–gels with and without Celite® at two protein loading levels) were compared in terms of their immobilized protein content, conversion of methanol to methyl oleate, lipase activity, long term stability, and glycerol–water adsorption. The Celite® R632 sol–gel with high protein loading achieved the maximum conversion in the 6-h reaction period (90%). A drying step was found to be advantageous prior to the reaction, and the absorption of glycerol–water on the Celite® was only found to be significant at high levels of glycerol. The material was found to be very stable upon storage at 4 °C for up to 1.5 years, losing only about 15% of its percent conversion capacity per year. Based on this study, the supported immobilization technique shows significant potential as a novel catalyst for biodiesel production.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► Diatomaceous earth supported sol–gels are investigated to produce biodiesel. ► Conversion and stability are used to evaluate the performance of Celite® sol–gels. ► A pre-drying step increased the achievable conversion of the immobilized lipase. ► The material produced lost little conversion capacity after 1.5 years of storage. ► This immobilization technique shows good potential as a novel biodiesel catalyst.