Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
70284 | Journal of Molecular Catalysis B: Enzymatic | 2010 | 6 Pages |
A novel styrene monooxygenase (SMO) was isolated from Pseudomonas sp. LQ26, a styrene degrader from activated sludge. Sequence alignment demonstrated that it was the most distant member of all SMOs originating from the genus of Pseudomonas. The substrate spectrum of this enzyme extended beyond typical SMO substrates to 1-allylbenzene analogues, previously reported as non-substrates for the SMO from Pseudomonas fluorescens ST. The results demonstrate for the first time the asymmetric epoxidation of both conjugated and unconjugated alkenes catalyzed by SMO and suggest that a much broader substrate spectrum is expected for SMOs.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideResearch highlights▶ Styrene monooxygenase StyAB2 is isolated from Pseudomonas sp. LQ26. ▶ Both conjugated and unconjugated alkenes serve as substrates. ▶ Enantiomeric excesses of up to >99% are achieved for conjugated alkenes. ▶ Enantiomeric excesses of up to 86% are achieved for unconjugated alkenes.