Rapid and efficient gas chromatographic method for measuring the kinetics of lipase-catalyzed transesterification of phosphatidylcholine

Both n-3 polyunsaturated fatty acids (n-3 PUFA) and phospholipids have a lot of special functions on human body. n-3 PUFA includes α-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Among them, ALA is the precursor of EPA and DHA. The synthesis of ALA-containing phospholipids can solve the problems of low content of EPA and DHA in phospholipids and show an increased double effect on human body. At a water activity of 0.33, a rapid and efficient method was used for kinetics of the transesterification of phosphatidylcholine (PC) with α-linolenic acid ethyl ester (ALAEE). The rate equation was obtained using palmitic acid ethyl ester (PAEE) as index for reaction rate and the reaction proved to proceed via a Ping-Pong mechanism without inhibition by both the substrates.

Graphical abstractA rapid and efficient method was used for kinetics of the transesterification of phosphatidylcholine (PC) with α-linolenic acid ethyl ester (ALAEE). A rate equation was obtained using palmitic acid ethyl ester (PAEE) as index for reaction rate. The reaction proved to proceed via a Ping-Pong mechanism without inhibition by both the substrates.Figure optionsDownload full-size imageDownload as PowerPoint slideResearch highlights► A rapid and efficient method was used for kinetics of the transesterification of phosphatidylcholine (PC). ► Using palmitic acid ethyl ester (PAEE) as index for reaction rate. ► The reaction mechanism of Ping-Pong from the PAEE point of view was confirmed. ► Kinetics of the transesterification of phosphatidylcholine (PC) with α-linolenic acid ethyl ester (ALAEE).