| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 70327 | Journal of Molecular Catalysis B: Enzymatic | 2011 | 7 Pages |
A successful recipe for the production of immobilized invertase/porous silicon layer with appropriate catalytic behavior for the sucrose hydrolysis reaction is presented. The procedure is based on support surface chemical oxidation, silanization, activation with glutaraldehyde and finally covalent bonding of the free enzyme to the functionalized surface. The catalytic behavior of the composite layer as a function of pH, temperature, and the current density applied in the porous silicon (PS) preparation is investigated. Interestingly, Vmax undergoes a substantial increase (ca. 30%) upon immobilization. The value of Km increases by a factor of 1.53 upon immobilization. The initial activity is still preserved up to 28 days while the free enzyme undergoes a 26% loss of activity after the same period. Based on the outcomes of this study, we believe that tailored PS layers may be used for the development of new bioreactors in which the active enzyme is immobilized on the internal walls and is not lost during the process.
Graphical abstractA successful recipe for the production of immobilized invertase/porous silicon layer with appropriate catalytic behavior for the sucrose hydrolysis reaction is presented. The procedure is based on support surface chemical oxidation, silanization, activation with glutaraldehyde and finally covalent bonding of the free enzyme to the functionalized surface. The catalytic behavior of the composite layer as a function of pH, temperature, and the current density applied in the porous silicon (PS) preparation is investigated. Interestingly, Vmax undergoes a substantial increase (ca. 30%) upon immobilization. The value of Km increases by a factor of 1.53 upon immobilization. The immobilization process enhances considerably the storage stability of the enzyme. Based on the outcomes of this study, we believe that tailored PS layers may be used for the development of new bioreactors in which the active enzyme is immobilized on the internal walls and is not lost during the process.Figure optionsDownload full-size imageDownload as PowerPoint slideResearch highlights► Invertase enzyme has been successfully immobilized on porous silicon. ► The immobilized invertase shows a high activity for the sucrose hydrolysis reaction. ► The value of Vmax increases 30% upon immobilization. ► The immobilization process enhances considerably the enzyme storage stability.
