| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 70359 | Journal of Molecular Catalysis B: Enzymatic | 2009 | 8 Pages |
The main objective of this work was to study the enzymatic synthesis of short chain ethyl esters, a group of relevant aroma molecules, by Fusarium solani pisi cutinase in an organic solvent media (iso-octane), and to assess the influence of different parameters on the reaction yield.Cutinase displayed high initial esterification rates in iso-octane, which amounted to 1.15 μmol min−1 mg−1 for ethyl butyrate (C4 acid chain) and 1.06 μmol min−1 mg−1 for ethyl valerate (C5 acid chain). High product yields, 84% for ethyl butyrate and 96% for ethyl valerate, were observed after 6 h of reaction, for an initial equimolar concentration of substrates (0.1 M).The highest product yield (97%) was observed for ethyl caproate (C6) synthesis, a compound which is a part of natural apple and pineapple flavour, for an alcohol:acid molar ratio of 2 (0.2 M ethanol concentration).Cutinase affinity for short chain length carboxylic acids (C4–C6) in ester synthesis in iso-octane confirmed previous observations in reversed micellar system.
