| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 70375 | Journal of Molecular Catalysis B: Enzymatic | 2011 | 5 Pages |
To combine the advantage of the oleaginous yeast Yarrowia lipolytica with the high activity of some fungal lipases for oily wastewater treatment, an effective lipase-displaying arming yeast was constructed using the flocculation functional domain of Saccharomyces cerevisiae as the protein anchor. To estimate the effect of the whole-cell oily wastewater treatment, the lipase-displaying arming yeast was added into an open activated sludge bioreactor. Within 72 h of whole-cell treatment, 96.9% of oil and 97.6% of chemical oxygen demand (COD) were removed, while only 87.1% of oil and 91.8% of COD were removed in control A (Y. lipolytica Polg was added), 45.1% of oil and 67.5% of COD were removed in control B (no cell was added) in 72 h. The lipase-displaying arming yeast exhibited remarkable oil removal and COD degradation effect compared with the control samples, exemplifying its application potential.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideHighlights► We have the high activity lipase displayed on Y. lipolytica cell surface. ► The whole cell lipase has been added into activated sludge directly for oily wastewater treatment. ► We combine the advantage of Y. lipolytica with the lipase for oily wastewater treatment. ► This study is a pioneer work for wastewater treatment.
