| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 70444 | Journal of Molecular Catalysis B: Enzymatic | 2011 | 7 Pages |
Six esterases isolated from sequence and structure-guided genome mining approaches were evaluated for the kinetic resolution of secondary and tertiary alcohols that find application in the fine chemical and pharmaceutical industries. Activity and enantioselectivity with E-values of up to 24 were determined towards a range of sterically demanding tertiary alcohol esters. Excellent enantioselectivity (E > 100) was also achieved in the hydrolysis of a less challenging secondary alcohol ester, menthyl acetate. These results highlight that these approaches can be used for the identification of novel esterases applicable to the preparation of commercially desirable alcohols.
Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slideResearch highlights► New esterases were isolated from sequence and structure-guided genome mining. ► Activity towards kinetic resolution of secondary and tertiary alcohols was targeted. ► Products formed find application in the fine chemical and pharmaceutical industries. ► Moderate E-values were determined towards sterically demanding substrates. ► Excellent enantioselectivity achieved (E > 100) in the hydrolysis of menthyl acetate.
