| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 70475 | Journal of Molecular Catalysis B: Enzymatic | 2010 | 5 Pages |
Abstract
Optimization of the growth conditions for maximum β-mannanase production in shake flasks by using recombinant Aspergillus sojae ATCC11906 (AsT1) was carried out by Box–Behnken design of response surface methodology. The highest β-mannanase activity on the fourth day of cultivation at 30 °C was obtained as 363 U/ml in the optimized medium consisting of 7% sugar beet molasses, 0.43% NH4NO3, 0.1% K2HPO4 and 0.05% MgSO4 (by weight per volume) at 207 rpm. On the sixth day of cultivation under the optimized conditions, the highest β-mannanase activity was achieved as 482 U/ml which is 1.4-fold of 352 U/ml activity found on glucose medium previously.
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Authors
Bengu Ozturk, Deniz Cekmecelioglu, Zumrut Begum Ogel,