Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7068229 | Bioresource Technology | 2018 | 7 Pages |
Abstract
Vibrational wave treatment has been used to increase proliferation of microalgae. When directly applied at large scale, however, turbulence can offset positive effects of vibration on microalgae proliferation. Moreover, severe hydrodynamic shear fields in the bioreactor decrease cell viability that detrimentally influence maximum yieldable biomass. In this study, vibration pretreatment (between 10-30â¯Hz and 0.15-0.45â¯G) was used to prime the cells for enhanced biomass. When exposed to 10â¯Hz at 0.15â¯G for 72â¯h and inoculated in baffled flasks of large shear fields (0.292â¯Pa for the average wall shear force (aveWSF) and 184â¯sâ1 for the average shear strain rate (aveSSR)), microalgae showed 27% increase in biomass as well as 39% increase in corresponding amount of heterologous protein (i.e. GFP-3HA). Our results show that stress primed microalgae with vibrations can lead to improved proliferation that results in increased biomass production at industrial scale bioprocesses.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Process Chemistry and Technology
Authors
Sang-Min Paik, EonSeon Jin, Sang Jun Sim, Noo Li Jeon,