Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
70722 | Journal of Molecular Catalysis B: Enzymatic | 2009 | 4 Pages |
A strain of Aspergillus oryzae WZ007 producing intracellular lipase with preference for the formation of the d-biotin intermediate lactone ((3aS,6aR)-tetrahydro-1,3-dibenzylhexahydro-1H-Furo[3,4-d]imidazole-2,4-dione) 1 was screened from soil samples. The microbial cells fermented by the strain were used as biocatalysts after lyophilization, to resolve the racemic biotin intermediate lactone 1 by enantioselective lactonization in organic solvents. The enantioselective lactonization afforded optically active lactone 1 with high enantiomeric excess (e.e. ≥ 98%) and conversion ratio (c ≥ 40%) when the biotransformation was carried out in dichloromethane. The e.e. of product was up to 99% after purification by inducing crystallization in acetone and basically satisfied the industrial application. The biosynthesis has been employed to produce kilograms supplies of optically active lactone 1 with high optical purity.