| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 70833 | Journal of Molecular Catalysis B: Enzymatic | 2008 | 6 Pages |
Abstract
The application of (R)-hydroxynitrile lyases ((R)-HNLs) enables a simple chemo-enzymatic approach towards (R)-pantolactone synthesis. For the first time, several new recombinant almond (R)-HNL isoenzymes were compared with native HNLs from different Prunus species with respect to cyanohydrin formation from hydroxypivalaldehyde providing the chiral key precursor in HNL based (R)-pantolactone synthesis. Recombinant PaHNL5 (R-selective hydroxynitrile lyase, isoenzyme 5, from Prunus amygdalus) surpasses all other tested natural and recombinant HNL variants. At low pH even very low amounts of crude enzyme catalysed stereoselective hydroxypivalaldehyde cyanohydrin formation in water based reaction systems.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Beate Pscheidt, Manuela Avi, Richard Gaisberger, Franz S. Hartner, Wolfgang Skranc, Anton Glieder,