| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 70989 | Journal of Molecular Catalysis B: Enzymatic | 2006 | 6 Pages |
Abstract
A putative alcohol oxidase (AO) from abdominal tips (ATs) of Manduca sexta virgin females was studied in a biphasic system hexane/aqueous phosphate buffer. The pH optimum closest to neutral range (6.8) and the temperature optimum closest to room temperature (25 ± 3 °C) were measured for the highest AO activity. AOs that are in intact membranes have long lifespans and may oxidize repeatedly. A high selectivity for primary alcohols of benzylic, saturated, and allylic type was observed. Neither the secondary alcohols nor the primary alcohols with bulky alkyl groups on C2-carbon are oxidized. This pronounced substrate specificity can be used for specific oxidation of alcohols in mixtures.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Anna Luxová, Aleš Svatoš,