| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 71022 | Journal of Molecular Catalysis B: Enzymatic | 2009 | 5 Pages |
Abstract
β-Glucosidase (BGL1) from Aspergillus oryzae was efficiently produced in recombinant A. oryzae using sodM promoter-mediated expression system. The yield of BGL1 was 960 mg/l in liquid culture, which is 20-fold higher than the yield of BGL1 produced using the yeast Saccharomyces cerevisiae. Recombinant BGL1 converted isoflavone glycosides into isoflavone aglycones more efficiently than β-glucosidase from almond. In addition, BGL1 produced isoflavone aglycones even in the presence of the insoluble form of isoflavone glycosides.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Ken Horii, Takashi Adachi, Tetsuya Matsuda, Tsutomu Tanaka, Hiroshi Sahara, Seiji Shibasaki, Chiaki Ogino, Yoji Hata, Mitsuyoshi Ueda, Akihiko Kondo,