| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 71105 | Journal of Molecular Catalysis B: Enzymatic | 2007 | 5 Pages |
Abstract
Cellobiohydrolase CBH I (Cel7A) from the filamentous fungus Trichoderma reesei (TrCBHI), which is a member of glycoside hydrolase family (GHF) 7, was expressed in Aspergillus oryzae. We found that the recombinant enzyme showed significant chitosanase activity, as well as cellulase activity, and acted in an endo-type manner on soluble polymeric substrate. Furthermore, another GHF7 CBH I from Aspergillus aculeatus (AaCBHI) expressed in A. oryzae also had chitosanase activity, while endoglucanase EG I (Cel7B) from T. reesei had no activity towards chitosan. To our knowledge, this is the first report of GHF7 enzymes possessing chitosanase activity.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Masakazu Ike, Yohane Ko, Kengo Yokoyama, Jun-Ichi Sumitani, Takashi Kawaguchi, Wataru Ogasawara, Hirofumi Okada, Yasushi Morikawa,