Article ID Journal Published Year Pages File Type
71235 Journal of Molecular Catalysis B: Enzymatic 2006 4 Pages PDF
Abstract

In this paper we describe a colorimetric method for the screening of nitrilases. When a buffered solution of CoCl2 is added to a nitrilase-catalyzed hydrolysis reaction, the ammonia product forms a complex with the cobalt ion resulting in a color change from light pink to yellow, which can readily be quantified using a spectrophotometer at 375 nm. This method has been demonstrated for both wild-type and evolved nitrilases.

Related Topics
Physical Sciences and Engineering Chemical Engineering Catalysis
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