| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 71235 | Journal of Molecular Catalysis B: Enzymatic | 2006 | 4 Pages |
Abstract
In this paper we describe a colorimetric method for the screening of nitrilases. When a buffered solution of CoCl2 is added to a nitrilase-catalyzed hydrolysis reaction, the ammonia product forms a complex with the cobalt ion resulting in a color change from light pink to yellow, which can readily be quantified using a spectrophotometer at 375 nm. This method has been demonstrated for both wild-type and evolved nitrilases.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Catalysis
Authors
Daniel R. Yazbeck, Paulo J. Durao, Zhiyi Xie, Junhua Tao,