Inactivation studies on native and silica gel non-homogeneous immobilized chloroperoxidase

A pH stability analysis was made, in the presence of tert-butyl hydroperoxide, of both immobilized and native chloroperoxidase obtained from Caldariomyces fumago and the inactivation constants (j1) evaluated. The native enzyme displays a uni-exponential decay, whereas for the immobilized enzyme a three exponential equation describes the time dependent enzyme inactivation.For immobilized enzyme, three-exponential equation describes the enzyme time-course inactivation. The obtained inactivation constants (j3 and K3) showed an increase in the stability of a fraction of the immobilized enzyme. This is probably due to a decrease of the affinity of the enzyme for the oxidant and not to a decrease in j3 values.