Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7139179 | Sensors and Actuators B: Chemical | 2018 | 36 Pages |
Abstract
Fluorescence resonance energy transfer (FRET), a near-field energy transfer from a fluorescent donor to a fluorescent acceptor within close proximity, has been widely used in biosensing. In this study, a rapid and universal sensor for the detection of ovalbumin (OVA) using nitrogen, oxygen, phosphoric co-doped CDs (NOP-CDs) and graphene oxide (GO) was established based on the FRET technology. First, novel NOP-CDs peak at 443â¯nm were synthesized by a one-pot hydrothermal method using egg powder and phosphorus acid. Then, in the absence of OVA, the fluorescence of OVA-antibody modified NOP-CDs was quenched by GO via electrostatic attraction and Ï-Ï stacking interaction due to the FRET effect. The introduced OVA competed to displace the GO by special binding with the anti-OVA, resulting in the recovery of the fluorescence. This sensor displayed a linear response to OVA from 0.5 to 15â¯Î¼g/mL with a detection limit of 153â¯ng/mL and was successfully applied to the detection of the OVA sample with recoveries ranging from 99.25 to 118.90%. In summary, the green and easy-to-synthesize nano-material was used in this method, the accurate detection results, and the simple process exhibited the great potential of this method for practical applications.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xuan Fu, Long Sheng, Yisha Yu, Meihu Ma, Zhaoxia Cai, Xi Huang,