A magnetic separation fluorescent aptasensor for highly sensitive detection of bisphenol A

Bisphenol A (BPA) is an environmental endocrine hormone that is commonly considered to cause endocrine disorders and even life-threatening. Herein, a magnetic separation fluorescent aptasensor for highly sensitive detection of BPA is proposed based on AHN-labeled aptamer and magnetic nanoparticles. The BPA aptamer can bind to BPA with high affinity and strong specificity. The magnetic nanoparticles not only can enrich the target to improve the sensitivity, but also can separate the target from a complex environment or a biological sample to reduce the signal of the interference substances. In the proposed protocol, the fluorescence intensity of the sensing system is varied with different concentrations of BPA. The aptasensor exhibits a liner range from 0.20 to 8.00 ng/mL for BPA with a detection limit of 0.047 ng/mL. In addition, the method shows an excellent selectivity, and can be effectively applied to the actual environmental water samples.