Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7140343 | Sensors and Actuators B: Chemical | 2018 | 4 Pages |
Abstract
The current work is a continuation of our research targeted the development of novel optical sensing technologies for detection of mycotoxins. The method of (TIRE) was developed in the last decade as a combination of spectroscopic ellisometry and SPR and was proved to be a highly sensitive analytical tool in bio-sensing particularly attractive for detection of low molecular weight analytes, such as mycotoxins. The use of aptamers as highly specific artificial molecular receptors to ochratoxin A (OTA) in conjunction with the method Total Internal Reflection Ellipsometry (TIRE) is reported here for the first time. Our results showed a possibility of label-free optical detection of OTA down to 0.01â¯ppb in concentration in direct assay with specific aptamer. The kinetics of aptamer/OTA binding was studied with dynamic TIRE spectral measurements and allowed evaluating the affinity constant KDâ¯=â¯1.8â¯10â8â¯Mol which is characteristic for highly specific aptamer/OTA binding.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ali Al Rubaye, Alexei Nabok, Gaelle Catanante, Jean-Louis Marty, Ezster Takacs, Andras Szekacs,